Pregnancy and labor (birth) are the most important periods in the lives of women. Hormonal, physical and psychiatric changes are effective on woman’s general health, mood, relationships and sexuality. The aim of our study was to determine the effects of pregnancy on sexual life in pregnant women followed up in primary care and it is a cross-sectional study. Pregnant women who have high risk pregnancy, who divorced from her husband, who use psychiatric or systemic drugs were excluded from the study. From 86 pregnant women who admitted to the Hatay Province Narlıca Family Health Center between 1 October-1 November 2011 period, 79 were included in the study. In women participating the study week of pregnancy was 22.6 ± 8.8 weeks, mean age was 24.6 ± 4.7 years, weight was 63.4 ± 9.7 kg, mean height was 160.7 ± 4.8 cm. Nineteen percent of pregnant women were in the first-trimester, 45.6% in the second and 35.4% were in the third trimester. 67.1% of pregnant women participating in the study were complaining from pain during sexual intercourse. With progress in the trimester of pregnancy, the number of pregnant women complaining from dyspareunia was increasing. While the frequency of sexual intercourse during the prepregnancy period was more than 2 times per week and 2 times per day in 77.3 % of cases, the rate was dropped to 49.4% during the pregnancy period. While there was no cases who cannot orgasm during the pre-pregnancy period, this proportion rose to 30% during the pregnancy period. In our comparison about average libido scores, the average libido score during the pre-pregnancy and pregnancy periods were 7.1 and 5.5, respectively. The average libido scores during pregnancy were significantly decreasing (p= 0.001). For the first, second and third trimester average libido scores were 6.2, 5.7, 5.0, respectively. As trimester progresses, the average libido score significantly decreases. Following the findings this study, during pregnancy period; the frequency of intercourse and orgasm are significantly decreasing while the partner starting the sexual intercourse and the rate of masturbation are remaining the same.